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Elisa assay vs western blot8/3/2023 ![]() Due to their strong and highly specific interaction with Strep-tagged targets, both generate signals with high sensitivity, avoiding the need for a secondary antibody. As viable alternative to antibodies, Strep-Tactin® and Strep-Tactin® XT with different fluorescent conjugates can be used as well. ![]() Strep-tagged or biotinylated proteins, StrepMAB-Classic or StrepMAB-Immo conjugated to different dyes can be applied for direct target labeling. While both, chromogenic or fluorescent labels are utilized for detection, fluorescent labeling is especially popular due to the possibility of multicolor staining of different targets. In both methods the sample fixation is essential to preserve the morphology and structure. The advantage of both techniques is the possibility of imaging proteins in their proper histological context, which enables the localization of cellular compartments and can be detected using fluorescence microscopy. ![]() In contrast to immunohistochemistry, in immunocytochemistry the focus is not on tissues but on cells. For more details, see: Recommendations for Test Performance and Interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease.Immunohistochemistry and immunocytochemistryĪmongst the immunostaining methods, immunohistochemistry is the most prominent one, in which whole tissues can be visualized, and thus, specific targets can be detected. Before CDC will recommend new tests, they must be cleared by the Food and Drug Administration (FDA). New tests may be developed as alternatives to one or both steps of the two-step process. Positive IgM results should be disregarded if the patient has been ill for more than 30 days.ĬDC supports the development of new tests Some tests give results for two types of antibody, IgM and IgG.Infection with other diseases, including some tickborne diseases, or some viral, bacterial, or autoimmune diseases, can result in false positive test results.Antibodies normally persist in the blood for months or even years after the infection is gone therefore, the test cannot be used to determine cure.Antibodies can take several weeks to develop, so patients may test negative if infected only recently.Most Lyme disease tests are designed to detect antibodies made by the body in response to infection.The overall result is positive only when the first test is positive (or equivocal) and the second test is positive (or for some tests equivocal). If the first step is positive or indeterminate (sometimes called “equivocal”), the second step should be performed. If this first step is negative, no further testing is recommended. Both steps are required and can be done using the same blood sample. ![]()
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